Question 112 marks
Question
There are similarities and differences between the structure of a typical plant cell and a typical animal cell. (a) The nucleus of plant cells and animal cells contains chromosomes. In interphase of the cell cycle, individual chromosomes are present but cannot be seen. The chromosome material is known as chromatin. (i) Changes occur in interphase, which result in a difference between the chromatin in the G1 phase compared with the chromatin in the G2 phase. State and explain the difference in chromatin in the G1 phase compared with chromatin in the G2 phase. …… [2] (ii) Describe the features of a nucleus, other than containing chromatin. You may use the space below the lines for a diagram. [3] * 0000800000003 * DFD 3 (b) Starch, cellulose and pectins are polysaccharides found in plant cells but not in animal cells. Pectins are complex polysaccharides that are found in the cell wall. (i) Describe the structural features of starch that are different from the structural features of cellulose. …… [4] (ii) Cell wall pectins can vary in different plant cell types and in different stages of cell development. Pectin molecules are released from cells as basic structures and then modified within the cell wall by adding side chains. RG‑I is a pectin molecule with a variable structure. The basic structure is a repeated disaccharide made from two different monosaccharides. RG‑I has three different side chains that can be added in different positions. Monoclonal antibodies (mAbs) are used to investigate the structure, location and role of cell wall pectins. Suggest and explain why scientists need to use a number of different monoclonal antibodies when investigating a pectin such as RG‑I. …… [3] [Total: 12] * 0000800000004 * DFD 4
Mark scheme
1(a)(i) explanation 2 semi-conservative replication / DNA replication / S phase, has occurred ; R if replication stated to occur in, G 1 / G2 I chromatin replicates difference between chromatin in G1 and G2: (allow ecf from mp1 if stated replication occurs in G2) Any one from: double the / doubling in, quantity / number / amount, of, chromatin / DNA ; A chromatin has doubled sister / two identical, chromatids (formed, instead of one structure) ; A two chromatids joined at the centromere (in G2) (each) chromosome with 1 DNA molecule (in G1) has 2 DNA molecules (in G2) ; allow double, helix/ helices for molecule(s) treat as neutral ref. to chromatin / chromosome, state / behaviour e.g. decondensed / diffuse 1(a)(ii) any three from: check diagram to see if points can be awarded 3 description of nucleus as having any two listed structures ; two from: nuclear envelope, nuclear pore(s), nucleolus, nucleoplasm correct spelling detail of nuclear envelope ; e.g. two membranes / double membrane accept from diagram encloses / protects, chromatin / DNA / chromosomes / genetic information / genetic material outer membrane continuous with rough endoplasmic reticulum outer membrane with ribosomes (on outer surface) controls exit and entry of substances if nuclear envelope not stated, A the nucleus is surrounded by a double membrane or a double membrane encloses, chromatin / AW or (it is) double-membrane bound / bound by a double membrane detail of nuclear pore(s) ; e.g. allows (m)RNA to, pass through / leave / AW (nucleus) allows ribosomal subunits to leave (nucleus) allows entry of proteins prevents exit of DNA (small) channel / passage, through the nuclear envelope can be shown on a diagram, with a label detail of nucleolus ; e.g. spherical can be shown as circle on diagram, labelled nucleolus one / can be more than one synthesis of, rRNA / ribosomal subunits A ribosomes densely staining / dense region / condensed region allow shaded in on a diagram, labelled nucleolus AVP ; e.g. nucleoplasm is fluid surrounding chromatin 1(b)(i) R the mp if feature of cellulose stated and, is incorrect / is not neutral 4 for mps 1, 3, 4 can accept ‘cellulose does not have ..’ any four from: starch has 1 (monomer of) alpha glucose / α-glucose / α-1,4 (glycosidic) bond ; I glucose / glycosidic bond / 1, 4 bond (all shared with cellulose) 2 monomers / glucose, orientated the same way / AW ; ora for cellulose i.e. monomers rotated 180° to each other I cellulose / cellulose molecule, rotates 180° 3 made of, two different polysaccharides / two different polymers / amylose and amylopectin ; 4 amylopectin / starch, has (α-1,4 and) α-1,6 (glycosidic) bonds ; A 1-6 if alpha noted elsewhere A link / linkage, for bonds R ‘can have α-1,4 or α-1,6 bonds’ 5 amylose, helical / coiled R α-helix or amylopectin branched ; if both are stated, allow mp3 as well A starch, is branched / has branching (v cellulose, not branched / linear) R ‘starch can be helical or branched’ 6 no, hydrogen / H, bonds, to hold together / between / AW, molecules / polysaccharides ; A cellulose has ……. 7 AVP ; e.g. forms, starch, grains / granules A ‘cellulose does not have ..’ does not have microfibrils / fibrils / fibres A ‘cellulose has..’ 1(b)(ii) 1 (different) mAbs have, different / specific, antigen binding sites / variable regions ; I active site 3 2 (each) mAb has complementary shape for, binding / AW, antigen (combining with / attaching to) or mAbs are / a mAB is, specific / complementary, to particular, antigen(s) ; 3 idea that a (particular) pectin / RG-I , has different, shapes / conformations / antigens, in its structure ; 4 idea of (using) different / specific, (sets / combinations, of) mAbs to, recognise / identify / locate / use for, different pectins / types of RG-I / a particular RG-I ; 5 ref. to studying development e.g ; changes occurring / side chains added, during development need different mAbs some mAbs may be specific to side chains not yet added can help to show when modifications occur 6 AVP ; e.g. reason to use more than one mAB • some mAbs may bind to more than one type of pectin • other cell wall components, may have, similar structures / same side chains • some areas of RG-I may be out of reach for a specific mAb • can, obtain information about / investigate, shape / structure of the particular pectin