Biology 0610/52 February/March 2023: question paper and mark scheme
Cambridge IGCSE Biology (0610), Paper 5 Practical Test, February/March 2023. Read both free below, or download them. Done the paper? Get it marked by AI against this mark scheme.
Potato cells absorb methylene blue dye when the potato tissue is placed into the dye solution.
If the dyed potato cells are placed in water the dye will diffuse into the water.
You are going to investigate the effect of temperature on the diffusion of methylene blue dye from dyed potato cells.
Read all the instructions but DO NOT DO THEM until you have drawn a table for your results in the space provided in 1(a)(ii).
You should use the safety equipment provided while you are doing the practical work.
Step 1 Raise your hand when you are ready for hot water to be put into the beaker labelled hot water.
Step 2 Measure the temperature of the water in the beaker labelled hot water.
Record this measurement in the space provided in 1(a)(i).
Step 3 Label one test-tube C, one test-tube W and one test-tube H.
Step 4 Draw a line 5 cm from the base of each test-tube, as shown in Fig. 1.1. Place the three test-tubes in the test-tube rack.
line drawn
5 cm
Fig. 1.1
Step 5 Fill test-tube C with cold water up to the line drawn in step 4. Put test-tube C into the beaker labelled cold water.
Step 6 Fill test-tube W with warm water up to the line drawn in step 4. Put test-tube W into the beaker labelled warm water.
Step 7 Fill test-tube H with hot water up to the line drawn in step 4. Put test-tube H into the beaker labelled hot water.
Step 8 You are provided with three potato cylinders in the beaker labelled P. They have been soaked in methylene blue solution and then rinsed.
Place the three potato cylinders on the white tile and cut the three potato cylinders to approximately 2 cm in length.
Step 9 Place one of the potato cylinders from step 8 into each test-tube.
Step 10 Start the stop-clock. Leave the test-tubes for 15 minutes.
Continue with the other questions while you are waiting.
Step 11 After 15 minutes, measure the temperature of the water in the beaker labelled hot water again.
Record this measurement in the space provided in 1(a)(i).
(a) (i) Record the temperatures you measured in step 2 and step 11.
Include the unit.
water temperature in the beaker labelled hot water in step 2 ……
water temperature in the beaker labelled hot water in step 11 ……
[2]
Step 12 Remove the test-tubes from the beakers and shake all the test-tubes for 10 seconds.
Place the test-tubes into the test-tube rack.
Step 13 Hold the piece of white card behind the test-tubes to help you observe the contents of the test-tubes.
Observe the intensity of the blue colour of the liquid in test-tubes C, W and H.
Record your observations in your table in 1(a)(ii).
(ii) Prepare a table to record your results.
[3]
(iii) State a conclusion for your results.
…… [1]
(iv) Identify one possible source of error in step 8.
…… [1]
(v) Identify one safety hazard in the investigation.
…… [1]
(vi) Your measurements in 1(a)(i) may suggest that the temperature of the water during the investigation was a source of error.
Suggest an improvement to reduce this type of error.
…… [1]
(b) A student investigated the effect of surface area on diffusion.
The student used this method:
• Cut four cubes from a potato. Each cube should be a different size.
• Put the potato cubes into a methylene blue solution for 24 hours.
• After 24 hours, remove the potato cubes from the solution and rinse them in cold water.
• Fill four test-tubes with water. Place one potato cube into each test-tube. Leave the test-tubes for 15 minutes.
• Some of the methylene blue dye will diffuse out of the potato cube into the water during the 15 minutes. Shine a light through the water in the test-tube after
15 minutes.
• Measure the percentage of light that is absorbed by the methylene blue dye in the water in each test-tube.
• The higher the concentration of methylene blue dye in the water the greater the percentage of light absorbed.
(i) State the independent variable and the dependent variable in the investigation described in 1(b).
independent variable ……
dependent variable ……
[2]
(ii) State one variable that was kept constant in the investigation described in 1(b).
…… [1]
(iii) Fig. 1.2 is a graph showing the student’s results.
100
90
80
70
60 percentage of light absorbed 50 by the methylene blue dye 40
30
20
10
0
0 5 10 15 20 25 30 surface area of the potato cube / cm2
Fig. 1.2
Using Fig. 1.2, estimate the percentage of light absorbed by the methylene blue dye when the surface area of the potato cube is 18 cm2.
Show on Fig. 1.2 how you obtained your estimate.
[2]
[Total: 14]
Mark scheme
1(a)(i) initial temperature higher than final temperature ; 2
°C ;
1(a)(ii) table drawn with minimum of two columns and a header 3
line ;
appropriate, column / row, headings and correct data
recorded in data cells ;
data recorded with suitable trend ; MP3 check supervisor’s report
1(a)(iii) as temperature increases (the rate of) diffusion is, faster ; 1
ora
1(a)(iv) differences in (the volume / concentration of) dye in the 1
cylinders / differences in, rinsing / soaking / AW ;
differences in, length / size, of the (potato) cylinders ;
1(a)(v) (use of a) scalpel / knife (to cut the cylinder) ; 1
hot (water) ;
AVP ; (use of) methylene blue / allergy from potato
1(a)(vi) use of a thermostatically controlled water-bath / method of 1
maintaining temperature in a water-bath described ;
1(b)(i) independent variable: surface area of, 2
cube / pieces / potato / AW ;
dependent variable: percentage of light (absorbed) ;
1(b)(ii) tissue / potato ; 1
(length of) time (soaking in methylene blue / in water in test-
tube) ;
type of dye ;
1(b)(iii) indication on graph ; 2
67 ± 1(%) ; ecf from incorrect indication on graph
(a) A scientist investigated the nutritional content of fruit juice.
(i) State the name of the reagent or solution that would be used to test the fruit juice for starch.
…… [1]
(ii) State the name of the reagent or solution that would be used to test the fruit juice for reducing sugars.
…… [1]
(iii) A sample of fruit juice was tested for protein.
State the result of a positive test.
…… [1]
(b) Fruit juice contains vitamin C.
Plan an investigation to determine the effect of temperature on vitamin C concentration in fruit juice.
…… [6]
(c) Scientists wanted to find out if drinking beetroot juice lowers blood pressure.
Two groups of men had their systolic blood pressure measured. Group 1 drank 500 cm3 of beetroot juice and group 2 drank 500 cm3 of apple juice.
After six hours their systolic blood pressures were measured again.
The results are shown in Table 2.1.
Table 2.1
mean systolic blood group pressure / mm Hg before drinking after six hours
1 132.4 127.4
2 131.3 132.1
(i) Using the data in Table 2.1, calculate the percentage change in mean systolic blood pressure for group 1.
Give your answer to one decimal place.
Space for working.
[3]
(ii) State one factor that was kept constant in the investigation described in 2(c).
…… [1]
(d) Fig. 2.1 is a photomicrograph of a section through a bronchiole in a human lung.
P
Q
magnification ×130
Fig. 2.1
(i) Line PQ represents the width of the bronchiole.
Measure the length of line PQ in Fig. 2.1.
length of line PQ …… mm
Calculate the actual width of the bronchiole using the formula and your measurement. length of line PQ magnification = actual width of the bronchiole
Give your answer to two significant figures.
Space for working.
[3]
(ii) Make a large drawing of the layers of tissue in the bronchiole shown in Fig. 2.1.
Do not draw individual cells.
[4]
(e) A scientist investigated the effect of exercise on breathing rate and heart rate while running.
They measured the percentage increase in breathing rate and heart rate from the resting rates during a four-minute run.
The results are shown in Table 2.2.
Table 2.2
percentage increase from resting rates time / s breathing rate heart rate
0 0 0
60 92 21
120 123 40
180 135 59
240 142 77
(i) Using all the data in Table 2.2, plot a line graph on the grid of the percentage increase from resting rate against time.
You will need to plot breathing rate and heart rate as two separate lines on your graph.
Include a key.
[5]
(ii) State a conclusion for the data shown in your graph.
…… [1]
[Total: 26]
Mark scheme
2(a)(i) iodine (solution) ; 1
2(a)(ii) Benedict’s (solution) ; 1
2(a)(iii) lilac / purple / AW ; 1
2(b) independent variable: 6
1 at least two, different temperatures ;
dependent variable:
2 volume / number of drops, of DCPIP added (to a
constant volume of fruit juice) until colour change OR
volume / number of drops, of fruit juice added (to a
constant volume of DCPIP), until colour change / blue
to, colourless / colour of fruit juice ;
method ;;;
3 equilibration to reach temperature (before titration)
4 method of measuring volume of added, DCPIP / fruit
juice (to align with the dependant variable)
5 shaking / stirring, after each added drop
6 method to check end point
constant variables ;;
7,8 type of fruit juice / (starting) volume, fruit juice / DCPIP
(to align with the stated method) / (starting)
concentration DCPIP
9 total of three or more replicates / repeat investigation
two or more times ;
10 appropriate safety precaution ;
2(c)(i) (–)3.8 (%) ;;; 3 MP1 correct data selected i.e. 132.4 and 127.4
MP2 correct calculation
MP3 correct rounding to one decimal place
ecf from MP1 or MP2
2(c)(ii) volume of juice ; 1
sex of participant / all males ;
time gap between blood pressure measurements ;
AVP ;
2(d)(i) length of line PQ: 96 ±1 (mm) ; 3 MP1 correct measurement of line PQ
0.74 ± 0.01 ;; MP2 correct calculation
MP3 correct rounding to two significant figures
ecf from MP1 or MP2
2(d)(ii) outline: single clear line and no shading ; 4
size larger than image ;
details ;;
• number of invaginations of bronchiole lining
• muscle layer shown break in correct position
2(e)(i) 1 axes labelled with units ; 5
2 even scale that occupies at least half the grid in both
directions ;
3 points plotted accurately ± half a small square ;
4 two separate lines drawn joining plot points either dot to
dot or with a smooth curve ;
5 key that clearly and correctly identifies each line
plotted ;
2(e)(ii) as length of time of exercise / running, increases the 1
(percentage increase in), breathing / heart, rate increases ;
the percentage increase in breathing rate is higher than the
percentage increase in heart rate, when, running /
exercising ;
AVP ;
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On this page, free: the mark scheme for IGCSE Biology 0610/52, February/March 2023 (0610_m23_ms_52), and the question paper (0610_m23_qp_52), to read or download, with each question's marking points written out underneath.
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Biology 0610/52 for February/March 2023 is out of 40 marks, with 1 hour 15 minutes to answer it, across 2 questions.
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