Biology 0610/51 October/November 2020: question paper and mark scheme

Question

The enzyme lipase catalyses the break-down of fats into fatty acids and glycerol. You are going to investigate how the concentration of lipase affects the break-down of fat in milk. An increase in the concentration of fatty acids will change the pH of the milk. You are going to use the pH indicator bromothymol blue to determine the pH of the milk and lipase solution during the investigation. Table 1.1 shows the colour of bromothymol blue indicator at different pH values. Table 1.1 pH 6 7 8 colour yellow green blue Read all the instructions but DO NOT CARRY THEM OUT until you have drawn a table for your results in the space provided in Question 1(a)(ii). You should use the safety equipment provided while you are carrying out the practical work. (a) Step 1 Label four test-tubes L1, L2, L3 and L4. Step 2 Make solutions containing different concentrations of lipase. Use the volumes of 2% lipase solution and distilled water as shown in Table 1.2. Table 1.2 volume of volume of distilled percentage concentration test-tube 2% lipase / cm3 water / cm3 of lipase solution L1 3.00 0.00 2.0 L2 1.50 1.50 1.0 L3 0.75 2.25 L4 0.00 3.00 0.0 (i) Calculate the percentage concentration of lipase solution in test-tube L3 using the information in Table 1.2. Space for working. ……% [1] Step 3 Label another four test-tubes M1, M2, M3 and M4. Step 4 To each of test-tubes M1, M2, M3 and M4 add: • 5 drops of bromothymol blue indicator • 2 cm3 of sodium carbonate solution • 2 cm3 of milk. Step 5 Raise your hand when you are ready for warm water to be put into the beaker labelled water-bath. Step 6 Put all eight test-tubes into the water-bath and leave for five minutes. Step 7 After five minutes remove test-tubes M1 and L1 from the water-bath and place in a test-tube rack. Step 8 Pour the solution in test-tube M1 into test-tube L1. Start a stop-clock. Step 9 Observe the colour of the bromothymol blue indicator in test-tube L1 and record the time at which it becomes yellow. If the colour has not changed to yellow in five minutes, stop observing and record the result as >300. Step 10 Repeat steps 7, 8 and 9 for test-tubes M2 and L2, M3 and L3, M4 and L4. Record your results in the table you have prepared in 1(a)(ii). You should record your results in seconds. (ii) Prepare a table to record your results. [4] (iii) State a conclusion for your results. …… [1] (b) (i) Identify the control in this investigation and explain why a control was used. control …… explanation …… [2] (ii) Using the information in Table 1.1 and your results, estimate the pH values in test-tube L1 and test-tube L4 at the end of the investigation. L1 …… L4 …… [1] (iii) State two variables that were kept constant in this investigation. 1 …… 2 …… [2] (iv) Suggest why all of the test-tubes were placed into a water-bath for five minutes, in step 6, before mixing their contents. …… [1] (v) State the potential source of error in step 9. …… [1] (c) Describe how you would safely test lipase for the presence of protein and state the result of a positive test. method …… positive result …… safety precaution …… [3] (d) The average temperature of the human body is 37 °C. Humans produce lipase for fat digestion. A student thought that lipase would work best at human body temperature. Plan an investigation to find out if 37 °C is the optimum (best) temperature for lipase activity. …… [6] [Total: 22]

Question

(a) Fig. 2.1 is a labelled diagram of the parts of a flower. petal stigma anther stamen style carpel filament ovary sepal Fig. 2.1 Fig. 2.2 is a photograph showing the parts of a flower that have been separated. A B C Fig. 2.2 Complete Table 2.1 using the information in Fig. 2.1 and Fig. 2.2 by stating the: • names of flower parts A, B and C • number of each of the flower parts A, B and C visible in Fig. 2.2. Table 2.1 letter on Fig. 2.2 name of flower part number visible A B C [3] (b) Fig. 2.3 shows a photograph of a germinating pollen grain. pollen tube X pollen grain Y magnification ×350 Fig. 2.3 (i) Make a large drawing of the germinating pollen grain shown in Fig. 2.3. Label the pollen tube. [5] (ii) Measure the length of line XY on Fig. 2.3. length of line XY …… mm Calculate the actual length of the pollen grain in Fig. 2.3 using the formula. length of XY on Fig. 2.3 magnification = actual length of XY Include the unit. Space for working. [3] (c) Some students collected pollen from the anthers of flowers to investigate the effect of two different solutions, S1 and S2, on the germination of pollen. Two microscope slides were prepared. Slide one had 210 pollen grains and two drops of solution S1. Slide two had 250 pollen grains and two drops of solution S2. Every 10 minutes the students counted and recorded the number of pollen grains that had germinated. The percentage of pollen grains that had germinated was calculated. Fig. 2.4 shows a drawing of the pollen grains as seen with a light microscope. germinated pollen grain ungerminated pollen grain Fig. 2.4 The results of the investigation are shown in Table 2.2. Table 2.2 percentage germination time / minutes 10 20 30 40 50 60 solution S1 5 18 26 38 51 51 solution S2 3 8 18 28 36 51 (i) State two conclusions for these results. 1 …… 2 …… [2] (ii) The results in Table 2.2 are shown as percentages rather than as the actual number of germinated pollen grains. Explain why this enables a valid comparison to be made between the results for S1 and S2. …… [1] (iii) Describe how the percentage germination in Table 2.2 was calculated. …… [2] (d) The students prepared another three slides using solutions A, B and C and left them for 60 minutes. They measured the length of the pollen tubes in 20 germinated pollen grains. The results are shown in Table 2.3. Table 2.3 solution average length of pollen tube / µm A 190 B 220 C 265 Fig. 2.5 shows the grid that the students used to plot a graph of their results. average length of pollen tube / μm A solution Fig. 2.5 Use the information in Table 2.3 to complete the graph in Fig. 2.5 by: • adding the scale for the y-axis • plotting the bars for solutions B and C. [2] [Total: 18]