Biology 0610/53 October/November 2022: question paper and mark scheme
Cambridge IGCSE Biology (0610), Paper 5 Practical Test, October/November 2022. Read both free below, or download them. Done the paper? Get it marked by AI against this mark scheme.
You are going to investigate the effect of glucose solution concentration on the rate of respiration in a suspension of yeast cells.
The word equation for aerobic respiration is:
glucose + oxygen water + carbon dioxide
Read all the instructions but DO NOT DO THEM until you have drawn a table for your results in the space provided in 1(a)(iii).
You should use the safety equipment provided while you are doing the practical work.
Step 1 Label one test-tube A and a second test-tube B. Label the third test-tube water.
Step 2 Add 1 cm3 of the 0.1 mol per dm3 glucose solution to test-tube A.
Step 3 Add 4 cm3 of distilled water to test-tube A.
(a) (i) Use the information in step 2 and step 3 to calculate the concentration of glucose solution in test-tube A.
…… mol per dm3 [1]
Step 4 Use the glass rod to stir the yeast suspension.
Step 5 Use a syringe to put 10 cm3 of the yeast suspension into test-tube A. Use the glass rod to mix the contents of test-tube A.
Step 6 Raise your hand when you are ready for the hot water to be added to the beaker labelled water-bath.
Step 7 Measure the starting temperature of the water in the water-bath and record this measurement in the space provided in 1(a)(ii).
Step 8 Pour tap water into the test-tube labelled water and set up the apparatus as shown in
Fig. 1.1.
bung and delivery tube
test-tube A water test-tube, filled containing with tap water the yeast suspension and placed in the and glucose solution test-tube rack
water-bath containing bubbles of gas hot water will appear here
tap water
Fig. 1.1
Step 9 Once the apparatus is set up, leave it for two minutes. Bubbles of gas will start to appear at the end of the delivery tube.
Step 10 Start the stop-clock and count the number of bubbles produced in three minutes. Record this measurement in your table in 1(a)(iii).
Step 11 Remove test-tube A from the apparatus and place it in the beaker labelled waste.
Step 12 Put 5 cm3 of the 0.1 mol per dm3 glucose solution into test-tube B.
Step 13 Stir the yeast suspension again.
Step 14 Use a syringe to put 10 cm3 of the yeast suspension into test-tube B and mix the contents of the test-tube.
Step 15 Place test-tube B into the water-bath and connect the bung and delivery tube. Repeat step 9 and step 10.
Step 16 Measure the temperature of the water in the water-bath again and record this as the final temperature in the space provided in 1(a)(ii).
(ii) starting temperature of the water in the water-bath …… °C
final temperature of the water in the water-bath …… °C
[1]
(iii) Prepare a table to record your results.
[3]
(iv) Use your results to calculate the rate of bubble production for each of the test-tubes.
rate of bubble production for test-tube A …… bubbles per minute
rate of bubble production for test-tube B …… bubbles per minute
[1]
(v) State a conclusion for your results.
…… [1]
(vi) Identify the variable that you changed (the independent variable) in this investigation.
…… [1]
(vii) Explain why the temperature of the water in the water-bath is a source of error.
Suggest an improvement for this error.
explanation ……
improvement ……
[2]
(viii) Suggest why the yeast suspension was stirred in step 4 and step 13.
…… [1]
(ix) Suggest why the test-tube was left in the water-bath for two minutes in step 9.
…… [1]
(b) A student wanted to measure the volume of gas produced by respiring yeast cells in one minute.
Complete Fig. 1.2 by drawing and labelling the apparatus the student could use to do this.
Fig. 1.2
[2]
[Total: 14]
Mark scheme
1(a)(i) 0.02 (mol per dm3) ; 1
1(a)(ii) two temperatures recorded and final temperature is same or 1
lower than the starting temperature ;
1(a)(iii) table draw with headings underlined and minimum of two 3
columns and all headings correct ;
two results recorded ;
correct trend: test-tube A has fewer bubbles than test-tube B ; check supervisor’s report if trend not as expected
1(a)(iv) values from candidate’s table divided by 3 ; 1
1(a)(v) conclusion that matches results ; 1 e.g. at higher (glucose) concentrations (yeast) respires at
a higher rate
1(a)(vi) concentration of glucose ; 1
1(a)(vii) explanation: temperature decreased / changed / A starts at 2
different temperature to B / AW ;
improvement: use a thermostatically controlled / maintained
water-bath / top-up with warm water / use a Bunsen burner and
check the temperature with a thermometer / insulation / lid / do
test-tube A and test-tube B at the same time in same water-
bath ;
1(a)(viii) so that there was the same, amount / concentration / number, 1
of yeast (cells) in both test-tubes ;
1(a)(ix) any one from: 1
to reach temperature of water-bath ;
to equilibrate ;
to wait for, pressure to build up / volume to build up,
expansion / AW ;
to wait for gas to be pushed out of the delivery tube /AW ;
1(b) drawing that shows suitable method of collecting gas ; 2 e.g. drawing to show upturned vessel over water OR
(gas) syringe attached to delivery tube
indication of being able to measure volume of gas (from label e.g. label for, gas syringe / measuring cylinder,
or from drawing + label) ; or graduations drawn on the, beaker / test-tube / syringe
(a) Fig. 2.1 is a photograph of an inkcap mushroom. Mushrooms are a type of fungus that grow in fields and forests.
A B
magnification ×1.5
Fig. 2.1
(i) Make a large drawing of the inkcap mushroom shown in Fig. 2.1.
[4]
(ii) Line AB represents the width of the mushroom cap shown in Fig. 2.1.
Measure the length of line AB on Fig. 2.1.
length of line AB on Fig. 2.1 …… mm
Use your measurement and the formula to calculate the actual width of the mushroom cap.
length of line AB on Fig. 2.1 magnification = actual width of the mushroom cap
Give your answer to two significant figures.
Space for working.
…… mm
[3]
(b) In a study, the colour of the cap of mushrooms growing in different temperatures was recorded.
The colour was determined using the scale shown in Fig. 2.2.
cap colour
score 0 1 2 3 4 5 6 7 8
Fig. 2.2
The results are shown in Table 2.1.
Table 2.1
temperature / °C cap colour score
15 8
17 8
19 6
21 5
23 3
25 1
27 0
(i) Plot a line graph on the grid of the data in Table 2.1.
[4]
(ii) Using the information in your graph, describe the results of this study.
…… [2]
(iii) Fig. 2.3 shows the cap of a mushroom grown at a constant temperature.
Fig. 2.3
Using the scale in Fig. 2.2 and your graph, estimate the temperature that the mushroom in Fig. 2.3 was grown in.
Show on your graph how you obtained your estimate.
…… °C
[2]
(iv) Suggest why your answer in 2(b)(iii) can only be an estimate.
…… [1]
(v) All of the mushrooms used in the study were of the same species.
Suggest why it was important that they were all the same species.
…… [1]
(vi) State one variable, other than the species of mushroom, which should have been kept constant in this study.
…… [1]
(c) Some mushrooms contain vitamin C.
Describe the method you would use to test a sample of mushrooms for vitamin C.
[2]
[Total: 20]
Mark scheme
2(a)(i) outline clear and continuous, no shading ; 4
size - cap width greater than or equal to 65mm ;
detail 1: indication of white patch across the top of the cap
and cap drawn separate from stalk ;
detail 2: indication of textured stalk / rough edge to stalk ;
2(a)(ii) line A = 65 ±1 (mm) ; 3 MP1 correct measurement recorded
43 (mm) ;; MP2 correct answer to any number of significant figures
65 ÷ 1.5 = 43 (43.3333333333333)
MP3 answer correctly rounded to 2 sf
ecf MP2 and MP3 from incorrect measurements
ecf if cm used in mp1
if AB is 66 mm answer is 44 mm,
64 mm = 42.666666 or 43 mm
2(b)(i) axes labelled with unit for temperature ; 4
linear scale for plotted points to fill half or more of the grid in
both dimensions ;
all plotted points accurate to ± half a small square ;
suitable line drawn ;
2(b)(ii) any two from: 2
as temperature increases (mushroom) cap colour gets
lighter / score decreases / AW ; ora
use of data ;
no change, between 15–17°C ;
2(b)(iii) suitable temperature for mushroom of cap score 2 ; 2 A cap scores of between 1 and 3
expected value around 24°C
indication on graph of interpolation ; ecf from graph
2(b)(iv) subjective nature of colour score / reference to qualitative 1
nature of measurements AW / temperature based on a line of
best fit / between two known points / no colour measurement
was recorded at temperature in 3(b)(iii) / no intermediate
values ;
2(b)(v) different species may be different colours / may respond 1
differently to temperature / same species will all be same
colour / respond the same way, at the same temperature /AW ;
2(b)(vi) any one from: 1
(sun)light / pH / water / humidity / location / soil / time of day / ref.
to growing medium / nutrients / grow in same field / length of
time allowed to grow / age of mushroom cap / size of
mushroom / AW ;
2(c) any two from: 2
valid ref. to preparation to make an extract ; e.g. liquidise / cut up / put pieces in and shake / grind
add DCPIP ;
sample changes from blue to colourless if vitamin C present ;
Plan an investigation to find out how the volume of water added to germinating seeds affects the rate of germination.
…… [6]
Mark scheme
3 independent variable: 6
1 at least two different volumes of water added ;
dependent variable:
2 number of seeds germinated / time taken for seeds to
germinate ;
3, 4, 5 method ;;;
• method of maintaining temperature
• watering at regular intervals
• reference to placing seeds in trays / petri dish / test-
tube / planting in soil
• check seeds at regular intervals / at a set time period
• AVP, e.g. calculation of rate described
6, 7 constant variables ;;
• temperature
• type of seeds
• oxygen concentration
• pH
• type / amount of, soil / growing medium
• light (intensity)
8 repeat at least two times (three trials) ;
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On this page, free: the mark scheme for IGCSE Biology 0610/53, October/November 2022 (0610_w22_ms_53), and the question paper (0610_w22_qp_53), to read or download, with each question's marking points written out underneath.
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Biology 0610/53 for October/November 2022 is out of 40 marks, with 1 hour 15 minutes to answer it.
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