Biology 0610/22 October/November 2025: question paper and mark scheme
Cambridge IGCSE Biology (0610), Paper 2 Multiple Choice (Extended), October/November 2025. Read both free below, or download them. Done the paper? Get it marked by AI against this mark scheme.
The photographs show two different rats.
Rattus norvegicus Rattus rattus
Which statement about the rats is correct?
A The rats belong to the same genus.
B The rats belong to the same species.
C The rats can breed together to produce fertile offspring.
D The rats do not share any of the same features.
The diagrams show cells of organisms from three different kingdoms.
not to scale
1 2 3
Which row shows the kingdoms for the organism cells labelled 1, 2 and 3?
cell 1 cell 2 cell 3
A fungus protoctist prokaryote
B fungus prokaryote protoctist
C prokaryote fungus protoctist
D prokaryote protoctist fungus
The diagram shows a plant cell.
A biologist wants to find out the number of chromosomes it contains.
Which labelled part should be examined more closely?
B
C
A
D
The graph shows the changes in pH in the oesophagus, stomach and duodenum.
12
11
10
9
8 X
7 pH 6
5
4
3
2
1
0 oesophagus stomach duodenum region of the alimentary canal
Which substance causes the change in pH at the point labelled X?
A bile
B gastric juice
C glucagon
D trypsin
Which row names a small molecule and where that molecule is absorbed in the digestive system?
name of the small colon small molecule intestine
A amino acid key
B glucose = molecule is absorbed
C maltose = molecule is not absorbed
D water
A student cut one stalk of celery from a celery plant.
They put the celery stalk into a beaker of blue dye.
The celery stalk was removed from the blue dye after two hours and was cut into sections.
celery stalk
blue dye two hours
blue circles visible in the cut section
What is shown by the blue circles in the cut sections of celery stalk?
A position of the cortex cells
B position of the palisade cells
C position of the phloem cells
D position of the xylem vessels
Which process moves water from the surfaces of the mesophyll cells into the air spaces during transpiration?
A active transport
B circulation
C evaporation
D osmosis
The mass of water lost from a plant was investigated.
The leaves of the plant were covered with a type of grease that acts as a waterproof barrier.
The environmental conditions remained the same throughout the experiment.
The table shows the results of the investigation.
mass lost in treatment seven days / g
no grease applied 12.0 grease applied only to the upper surface of every leaf 8.7 grease applied to both surfaces of every leaf 0.0
What is the mean daily rate of water loss through the upper surface of the leaves?
A 0.47 g / day B 1.24 g / day C 1.71 g / day D 3.30 g / day
What is translocation?
A the movement of amino acids and water from sink to source
B the movement of starch and water from source to sink
C the movement of sucrose and amino acids from source to sink
D the movement of sucrose and mineral ions from sink to source
Which row describes what happens when breathing in?
external intercostal diaphragm muscles
A contracts contract
B contracts relax
C relaxes contract
D relaxes relax
A person exercised vigorously for one minute and then rested for one hour.
Which statements about lactic acid are correct?
1 During exercise, lactic acid is produced in the muscles and diffuses into the blood.
2 The blood transports lactic acid to the liver.
3 Lactic acid is broken down by anaerobic respiration.
A 1, 2 and 3 B 1 and 2 only C 2 and 3 only D 2 only
A person looks at a distant object in a dark area. The person then moves to a bright area and looks at a near object.
Which statement describes some of the changes that take place in the person’s eyes?
A The circular muscles of the iris contract, the pupil constricts, the ciliary muscles relax and the lens gets thicker.
B The radial muscles of the iris relax, the circular muscles of the iris contract, the pupil constricts and the lens gets thinner.
C The radial muscles of the iris relax, the pupil constricts, the ciliary muscles contract and the lens gets thicker.
D The radial muscles of the iris relax, the pupil dilates, the ciliary muscles relax and the lens gets thicker.
Which effect does adrenaline have on blood glucose concentration and pulse rate?
blood glucose pulse rate concentration
A decreases decreases
B decreases increases
C increases decreases
D increases increases
The diagram shows an experiment using two wheat shoot tips, X and Y.
after two days in even light
shoot tip removed
X Y X Y
Which statement is supported by the evidence provided by this experiment?
A Auxin moves through the plant by osmosis.
B Auxin is made in the shoot tip.
C Auxin is unequally distributed in response to light.
D Auxin inhibits cell elongation.
Which action would help to limit the development of antibiotic resistance in bacteria?
A Use antibiotics frequently to prevent infection by antibiotic-resistant bacteria.
B Use antibiotics only to treat bacterial infections.
C Use antibiotics to treat all types of infections.
D Use antibiotics to treat only viral infections.
What is an example of asexual reproduction?
A a single bacterium dividing to produce two genetically identical bacteria
B a mammal giving birth to four offspring
C a female bird laying six eggs which will hatch into the same species
D a tomato plant producing fruits which contain many seeds
The diagram shows parts of two different plants, 1 and 2, of the same species.
The transfer of pollen between the flowers of these two plants is shown by the arrows labelled
P and Q.
P
Q
1 2
Which arrows show cross-pollination, and which transfer of pollen would produce the most variation in the offspring?
transfer of pollen that produces the cross-pollination most variation in the offspring
A P only P
B P only Q
C P and Q P
D P and Q Q
Which substance is a hormone that is involved in the development and regulation of male secondary sexual characteristics?
A insulin
B glucagon
C lactase
D testosterone
Which statement describes the net direction of movement of a substance across the placenta?
A Carbon dioxide moves from the mother’s blood to the fetus’s blood.
B Glucose moves from the fetus’s blood to the mother’s blood.
C Glycogen moves from the mother’s blood to the fetus’s blood.
D Urea moves from the fetus’s blood to the mother’s blood.
Which methods can transmit HIV?
1 hugging a person infected with HIV
2 sharing a needle with a person infected with HIV
3 using a plate that a person infected with HIV has used
A 1, 2 and 3 B 1 only C 2 and 3 only D 2 only
Why are lymphocytes the only cells that produce antibody proteins?
A Lymphocytes express all the genes in the nucleus.
B Lymphocytes have twice as many genes as other cells.
C Other cells do not have the genes for antibodies.
D The genes for antibodies are only expressed in lymphocytes.
During protein synthesis, what is the function of the ribosome?
A It assembles amino acids in a chain.
B It carries a copy of a gene to the cytoplasm.
C It contains the code for the synthesis of a protein.
D It determines the order of bases in the protein.
The photograph shows a speckled chicken.
Speckled chickens have white feathers and black feathers.
When a chicken with only white feathers and a chicken with only black feathers are crossed, all of the offspring are speckled.
What would be the expected phenotypic ratio if a speckled chicken was crossed with a chicken with only white feathers?
A all offspring have only white feathers
B all offspring are speckled
C 1 white feathers only : 1 speckled
D 3 white feathers only : 1 speckled
What are stem cells?
A specialised cells that divide by meiosis to produce daughter cells
B specialised cells that divide by mitosis to produce daughter cells
C unspecialised cells that divide by meiosis to produce daughter cells
D unspecialised cells that divide by mitosis to produce daughter cells
What are features of hydrophytes which have leaves that float?
A long root system
B many stomata on upper surface of leaves
C hairy leaves
D small air spaces in the spongy mesophyll
Which unit could be used for the measurements needed to construct a pyramid of energy?
A kJ per g per m2
B kJ per kg per year
C kJ per m2 per year
D kJ per kg
Which process needs microorganisms to break down protein to produce ammonium ions?
A decomposition
B denitrification
C nitrification
D nitrogen fixation
The graph shows part of a growth curve for a bacterial population.
population size
time
What is not shown in the graph?
A the death phase
B the exponential phase
C the lag phase
D the stationary phase
What is an advantage of large scale monocultures?
A increased biodiversity
B increased crop yield
C increased susceptibility to disease
D increased variation in the crop plant
The graph shows the global emissions of carbon dioxide and methane from 1990 to 2015.
40 000
35 000
30 000 emissions 25 000 key
/ million 20 000 carbon dioxide tonnes 15 000 methane
10 000
5000
0
1990 1995 2000 2005 2010 2015 year
A student makes four statements about carbon dioxide and methane.
● Carbon dioxide and methane were the only sources of greenhouse gases produced in 1990.
● Carbon dioxide emissions continue to rise to the present day.
● Carbon dioxide has a greater effect on climate change than methane.
● Methane emissions were lower than carbon dioxide emissions between 1990 and
2015.
How many statements are supported by these data?
A 1 B 2 C 3 D 4
It's multiple choice: one mark for each correct answer, and nothing taken off for a wrong one. The mark scheme is the answer key.
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Where can I find the 0610/22 October/November 2025 mark scheme?+
On this page, free: the mark scheme for IGCSE Biology 0610/22, October/November 2025 (0610_w25_ms_22), and the question paper (0610_w25_qp_22), to read or download, with each question's marking points written out underneath.
How many marks is 0610/22 out of?+
Biology 0610/22 for October/November 2025 is out of 40 marks, with 45 minutes to answer it.
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Yes. Upload your answers to 0610/22/O/N/25 as a PDF or photos, and CIE Buddy marks every question against this paper's official mark scheme, using Cambridge's marking rules. You get your score, each lost mark explained on your own script, and the topics to revise. It's a guide, not an official Cambridge grade.