Biology 0610/22 May/June 2025: question paper and mark scheme
Cambridge IGCSE Biology (0610), Paper 2 Multiple Choice (Extended), May/June 2025. Read both free below, or download them. Done the paper? Get it marked by AI against this mark scheme.
The photograph shows an animal called a margay.
Four processes that occur in the margay are listed.
● excretion
● growth
● digestion
● respiration
How many of the processes that occur in the margay are characteristics of all living organisms?
A 1 B 2 C 3 D 4
Part of the base sequence of DNA from four species was determined.
species 1 TCAGGATT species 2 TGAGCAAT species 3 TGAGCAAG species 4 TGAGGATT
Which two species are the least closely related?
A 1 and 2 B 1 and 3 C 2 and 4 D 3 and 4
A single-celled organism has chloroplasts, a cell wall and a nucleus.
Which kingdom is this organism placed in?
A fungus
B plant
C prokaryote
D protoctist
A student measured the length of a structure using a microscope.
The magnification used was ×250.
The image size of the structure was 5.00 mm.
What was the actual length of the structure?
A 1.25 × 103 mm
B 1.25 × 103 μm
C 2.00 × 101 μm
D 2.00 × 10–5 μm
Which row shows the conditions when diffusion of oxygen into the blood is fastest?
alveolar surface concentration diffusion path area gradient distance
A high high short
B high low short
C low high long
D low low long
Uncooked potato cylinders of identical size were placed in four different liquids for two hours.
The different liquids are listed.
1 pure water
2 a sugar solution that was less concentrated than the contents of the potato cells
3 a sugar solution that was more concentrated than the contents of the potato cells
4 a sugar solution that was the same concentration as the contents of the potato cells
In which liquids will the potato cylinders increase in length?
A 1 and 2 B 1 and 3 C 2 and 4 D 3 and 4
What is the correct definition of enzymes?
A carbohydrates that act as biological catalysts
B carbohydrates that act as substrates
C proteins that act as biological catalysts
D proteins that act as substrates
A student investigated how the rate of photosynthesis is affected by carbon dioxide concentration, temperature and light intensity.
The graphs show the results.
2
rate of rate of photosynthesis photosynthesis
3
1
carbon dioxide temperature concentration
4 rate of photosynthesis
light intensity
What is a correct statement for these results?
A Carbon dioxide concentration is a limiting factor at 1.
B No enzymes are denatured at 3.
C Light intensity is a limiting factor at 4.
D Temperature is a limiting factor at 2.
The table shows the substrates and end products in four enzyme-controlled chemical reactions.
Which reaction was controlled by lipase?
substrate end product
A maltose starch
B oil fatty acids and glycerol
C protein amino acids
D starch maltose
What is the function of microvilli in the small intestine?
A conduction of electrical impulses
B increasing surface area for absorption
C movement of food particles
D movement of mucus
A student wanted to investigate the effect of humidity on transpiration. She set up two sets of apparatus with identical-sized plants of the same species.
plastic bag oil
water
tube X tube Y
The masses of the water in both tubes were measured at the start of the investigation and again after five days. The table shows the results of the investigation.
mass of the water mass of the water tube in the tube in the tube at the start / g at the end / g
X 40.3 34.6
Y 41.0 39.4
Which statement describes and explains these results?
A Transpiration in tube Y was higher than in tube X as the plastic bag decreased the humidity.
B Transpiration in tube Y was higher than in tube X as the plastic bag increased the humidity.
C Transpiration in tube Y was lower than in tube X as the plastic bag decreased the humidity.
D Transpiration in tube Y was lower than in tube X as the plastic bag increased the humidity.
What is an advantage of a double circulatory system?
A It allows the mixing of oxygenated and deoxygenated blood.
B It can keep blood pressure high in the lungs.
C Less carbon dioxide is removed from the body cells.
D Oxygen is supplied to body cells by blood at high pressure.
Which structure in the heart separates oxygenated and deoxygenated blood?
A atrioventricular valve
B muscle wall of left atrium
C semilunar valve
D septum
Which row shows the features of a vein?
relative thickness diameter of presence or of the wall to total the lumen absence of valves vessel diameter
A thick large absent
B thick small present
C thin small absent
D thin large present
Three statements about immunity are listed.
1 The transfer of antibodies from mother to baby in breast milk is an example of passive immunity.
2 Passive immunity results in long-term immunity because of the production of memory cells.
3 Active immunity is gained after vaccination with antigens.
Which statements are correct?
A 1, 2 and 3 B 1 and 2 only C 1 and 3 only D 2 and 3 only
The table shows the breathing rate and the average total volume of air breathed by a student when exercising for 20 minutes.
breathing rate average total volume time
/ breaths per of air breathed
/ minutes minute / cm3 per minute
0 12 5 000
5 12 9 000
10 12 15 000
15 18 20 000
20 22 25 000
Which conclusion can be made from the data in the table about the effect of exercise on breathing?
A Between 0 minutes and 10 minutes, there is no change to the depth of breathing.
B Between 0 minutes and 10 minutes, the depth of breathing increases.
C Between 10 minutes and 20 minutes, there is no change to the rate of breathing.
D Between 15 minutes and 20 minutes, the depth of breathing decreases.
After a race, athletes experience oxygen debt.
The diagram shows how the oxygen debt is removed.
lactic acid forms in the muscles
lactic acid moves in
X the blood to the liver
faster breathing supplies extra oxygen
What happens at X?
A aerobic respiration of glucose
B aerobic respiration of lactic acid
C anaerobic respiration of glucose
D anaerobic respiration of lactic acid
The diagram shows a vertical section through part of a human eye. A fly is coming nearer to the eye. The eye begins to focus the image of the fly on the retina.
Q
fly
R
S
How do the labelled parts of the diagram change?
Q R S
A contracts becomes thinner becomes tighter
B relaxes becomes fatter becomes slacker
C contracts becomes fatter becomes slacker
D relaxes becomes thinner becomes tighter
A young seedling is growing underground.
The shoot grows towards the surface of the ground.
The root grows away from the surface of the ground.
Which row describes the responses involved?
gravitropism phototropism root shoot root shoot
A yes yes no no
B yes no no yes
C no yes yes no
D no no yes yes
Clostridium difficile is a species of bacterium that is resistant to many antibiotics.
From 2002 to 2006, the number of people in one country infected with Clostridium difficile increased from 1300 to 6200 people.
What was the percentage increase in the number of people infected with Clostridium difficile from
2002 to 2006 to the nearest whole number?
A 21% B 27% C 79% D 377%
Which description of cross-pollination is correct?
A the transfer of pollen grains from the anther of one plant to the stigma on a different plant
B the transfer of pollen grains from the anther to the stigma on the same plant
C the transfer of pollen grains from the stigma of one plant to the anther on a different plant
D the transfer of pollen grains from the stigma to the anther on the same plant
Which row correctly links the named process with its description?
process description
A menstruation release of an egg
B menstruation shedding of the lining of the uterus
C ovulation fusion of nuclei
D ovulation start of puberty
The diagram shows the inheritance of a genetic condition in one family.
key
female with condition
female without condition
male with condition
male without condition
What can be concluded about this genetic condition?
A The alleles for the condition must be codominant.
B It is caused by a dominant allele.
C It is caused by recessive alleles.
D The alleles for the condition must be sex-linked.
How does artificial selection differ from natural selection?
A Artificial selection changes the characteristics of living things.
B Artificial selection is based on genetic variation.
C Artificial selection does not involve competition for resources.
D Artificial selection occurs over many generations.
Which diagram of a xerophyte is labelled correctly?
A B
waxy layer to waxy layer to stop water increase water evaporation evaporation
extensive root systems to extensive root systems to maximise water uptake maximise water uptake
C D
waxy layer to waxy layer to increase water stop water evaporation evaporation
restricted root systems to restricted root systems to minimise water uptake minimise water uptake
Which row describes the energy flow into and through a food chain that starts with a plant?
energy transferred energy entering between organisms a food chain in a food chain
A chemical chemical
B chemical heat
C light chemical
D light heat
In a food chain, zebras are large mammals that eat grass. The zebras have many insects living on them which suck their blood. Birds eat the insects.
The diagrams show some biological pyramids.
1 2 3 4
Which row identifies the types of pyramids for this food chain?
pyramid of pyramid of energy numbers
A 4 2
B 3 2
C 3 1
D 4 1
The diagram shows part of the nitrogen cycle.
organic material nitrogen gas
X ammonium ions nitrate ions
What is the process labelled X in the diagram?
A decomposition
B denitrification
C nitrification
D nitrogen fixation
The graph shows the number of water fleas in a newly-created pond.
Y
number of water fleas
time
Which event could be responsible for the shape of the graph at Y?
A the addition of extra oxygenating plants to the pond
B an increase in the birth rate of the water fleas
C an increase in the food supply for the water fleas
D the addition of predators which feed on water fleas
When managing fish stocks as a sustainable resource, what is a reason for harvesting only the larger fish of a particular species?
A Larger fish are easier to harvest.
B Larger fish sell at a higher price.
C Smaller fish are eaten by predators.
D Smaller fish are too young to breed.
Which features make bacteria useful in biotechnology?
1 They are unaffected by antibiotics.
2 They do not contain plasmids.
3 They make complex molecules.
4 They reproduce rapidly.
A 1 and 2 B 1 and 4 C 2 and 3 D 3 and 4
Answer
D
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On this page, free: the mark scheme for IGCSE Biology 0610/22, May/June 2025 (0610_s25_ms_22), and the question paper (0610_s25_qp_22), to read or download, with each question's marking points written out underneath.
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Biology 0610/22 for May/June 2025 is out of 40 marks, with 45 minutes to answer it.
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